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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
ELISpot Flex: Human IgG (HRP)
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ELISpot Flex: Human IgG (HRP)
ELISpot Flex: Human IgG (HRP) from Mabtech AB
Be the first to write a review!
Citations:
(15)
Supplier Page
Supplier Page from
Mabtech AB for
ELISpot Flex: Human IgG (HRP)
Description
Human IgG ELISpot Flex enables enumeration of B cells secreting total IgG as well as antigen-specific IgG. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP and polyclonal activators
Product Specs
Company Info
Citations
Reviews
Bench Tips
Product Specs
Item
ELISpot Flex: Human IgG (HRP)
Company
Mabtech AB
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
3850-2H
Quantity
for 4 plate
Applications
ELISpot
Reactivity
Human,Non-human primates
Detection Target
IgG
NCBI Full Gene Name
immunoglobulin heavy constant gamma 2 (G2m marker), immunoglobulin heavy constant gamma 3 (G3m marker), immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Gene Aliases
IgG3
Conjugate/Tag/Label
HRP
Type
Streptavidin-HRP
Format
without plates
Components
Capture mAbs (MT91/145),Biotinylated detection mAbs (MT78/145),Streptavidin-HRP,R848,Recombinant human IL-2
Add to Compare List
Mabtech AB
Box 1233
Nacka Strand
,
SE-131 28
Sweden
Phone
: +46 8 716 27 00
+46 8 716 27 01
Company Profile
Website:
http://www.mabtech.com
Citations (15)
(1)
The presence of baseline HBsAb-Specific B cells can predict HBsAg or HBeAg seroconversion of chronic hepatitis B on treatment
Emerging Microbes & Infections
December 8, 2023
Shengxia Yin, Yawen Wan, Rahma Issa, Yijia Zhu, Xiaoming Xu, Jiacheng Liu, Minxin Mao, Ming Li, Xin Tong, Chen Tian,
et al.
hours at 37°C with 5% CO2. Antibody-secreting cells were measured using the Human IgG
ELISpot
kit (
3850-2H
,
Mabtech
) after washing the cells with PBS and incubating them with biotin-labeled anti-IgG mAb and
(2)
High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays
Nature Biomedical Engineering
April 3, 2023
Saeid Ansaryan, Yen-Cheng Liu, Xiaokang Li, Augoustina Maria Economou, Christiane Sigrid Eberhardt, Camilla Jandus, Hatice Altug
Abcam
. SM-164 was obtained from LubioScience (HY-15989).
Mouse
and human IgG
ELISpot
kits (
3825-2A
and
3850-2H
) and biotinylated mouse IL-2 antibody (
3441-6-250
) were purchased from
Mabtech
.
FITC anti-human CD16
(3)
Evidence of premature lymphocyte aging in people with low anti-spike antibody levels after BNT162b2 vaccination
iScience
October 21, 2022
Yapei Huang, Juliana E. Shin, Alexander M. Xu, Changfu Yao, Sandy Joung, Min Wu, Ruan Zhang, Bongha Shin, Joslyn Foley, Simeon B. Mahov,
et al.
assay
,Abbott,N/A IgG (N) -
SARS-CoV-2
IgG
assay
,Abbott,N/A Human IgG ELISpotBASIC kit (HRP),
Mabtech
,Cat #:
3850-2H
"Human IgG (
SARS-CoV-2
, RBD) ALP",
Mabtech
,Cat #:
3850-4APW-R1-1
QIAseq Immune Repertoire RNA Library
(4)
Enhanced dengue vaccine virus replication and neutralizing antibody responses in immune primed rhesus macaques
npj Vaccines
December 1, 2021
Michael K. McCracken et al.
above.
ELISpot assay
Memory B cell
ELISpots were performed using the Human IgG
ELISpot
Basic kit (catalog no.
3850-2H
,
MabTech
). Cryopreserved PBMC were thawed and placed into culture for 6 days with R848 plus IL-2 in
(5)
Endoplasmic reticulum stress response and bile acid signatures associate with multi-strain seroresponsiveness during elderly influenza vaccination
iScience
September 24, 2021
Carre C, Wong G, Narang V, Tan C, Chong J, Chin HX, Xu W, Lu Y, Chua M, Poidinger M,
et al
.
incubator. Vaccine-specific IgG-secreting B cells were detected using a Human IgG
ELISpot
kit© (Cat:
3850-2H
;
Mabtech
, Nacka Strand, Sweden) according to the manufacturer's protocol.
ELISpot
plates were read using
(6)
Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infection
Nature communications
August 20, 2018
Song P, Zheng N, Liu Y, Tian C, Wu X, Ma X, Chen D, Zou X, Wang G, Wang H,
et al
.
(7)
Persistence of memory B-cell and T-cell responses to the quadrivalent HPV vaccine in HIV-infected children
AIDS (London, England)
April 24, 2018
Weinberg A, Huang S, Moscicki AB, Saah A, Levin MJ, IMPAACT P1085 Protocol Team..
(8)
Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients
Journal of immunology (Baltimore, Md. : 1950)
February 15, 2017
Bankó Z, Pozsgay J, Szili D, Tóth M, Gáti T, Nagy G, Rojkovich B, Sármay G.
(9)
DNA-MVA-protein vaccination of rhesus macaques induces HIV-specific immunity in mucosal-associated lymph nodes and functional antibodies
Vaccine
February 7, 2017
Chege GK, Burgers WA, Müller TL, Gray CM, Shephard EG, Barnett SW, Ferrari G, Montefiori D, Williamson C, Williamson AL.
(10)
Comparative phenotypical analysis of B cells in fresh and cryopreserved mononuclear cells from blood and tissue of rhesus macaques
Journal of immunological methods
June 1, 2016
Klippert A, Neumann B, Stahl-Hennig C.
(11)
The forkhead transcription factor FOXP1 represses human plasma cell differentiation
Blood
October 29, 2015
van Keimpema M, Grüneberg LJ, Mokry M, van Boxtel R, van Zelm MC, Coffer P, Pals ST, Spaargaren M.
(12)
A simple and safe technique for longitudinal bone marrow aspiration in cynomolgus and rhesus macaques
Journal of immunological methods
June 1, 2014
Spångberg M, Martinez P, Fredlund H, Karlsson Hedestam GB, Sundling C.
(13)
DNA/long peptide vaccination against conserved regions of SIV induces partial protection against SIVmac251 challenge
AIDS (London, England)
November 28, 2013
Koopman G, Beenhakker N, Nieuwenhuis I, Doxiadis G, Mooij P, Drijfhout JW, Koestler J, Hanke T, Fagrouch Z, Verschoor EJ,
et al
.
(14)
Optimization and qualification of a memory B-cell ELISpot for the detection of vaccine-induced memory responses in HIV vaccine trials
Journal of immunological methods
August 30, 2013
Walsh PN, Friedrich DP, Williams JA, Smith RJ, Stewart TL, Carter DK, Liao HX, McElrath MJ, Frahm N, NIAID HIV Vaccine Trials Network..
(15)
Optimization of a human IgG B-cell ELISpot assay for the analysis of vaccine-induced B-cell responses
Journal of immunological methods
May 31, 2013
Jahnmatz M, Kesa G, Netterlid E, Buisman AM, Thorstensson R, Ahlborg N.
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Reviews
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
ELISpot Flex: Human IgG (HRP) from Mabtech AB
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